TY - JOUR
T1 - Chemical Modifications of Electrospun Non-woven Hydroxypropyl Cellulose Fabrics for Immobilization of Aminoacylase-I
AU - Viswanathamurthi Periasamy, Kesavan Devarayan, Masakazu Hachisu, Jun Araki & Kousaku Ohkawa
JO - Journal of Fiber Bioengineering and Informatics
VL - 2
SP - 191
EP - 205
PY - 2012
DA - 2012/05
SN - 5
DO - http://doi.org/10.3993/jfbi06201208
UR - https://global-sci.org/intro/article_detail/jfbi/4874.html
KW - Aminoacylase
KW - Chemical modification
KW - Electrospinning
KW - Hydroxypropyl cellulose
KW - Immobilization
AB - Because of its wide solubility range, Hydroxypropyl Cellulose (HPC) is suitable for fabricating fine-fiber
materials via electrospinning. The resulting electrospun non-woven fabric (HPC-ESNW) requires an
appropriate post-spinning treatment for applications in aqueous environments. In the present study, we
examined the insolubilization of HPC-ESNW via cross-linking using bifunctional isocyanates.
Modification of the fine-fiber surfaces with NCO groups enables introduction of cationic functionalities;
we found that cationic Diethylaminoethyl (DEAE) groups are suitable for immobilization of aminoacylase-
I onto these fine-fiber surfaces under mild conditions. The NCO groups can be also converted to amino
groups, followed by activation with bifunctional N-hydroxysuccinimide (NHS) esters. The NHS-ESNW
can chemically bind the aminoacylase-I. Two kinds of immobilized enzyme were tested for stereospecific
recognition of a substrate and for immobilized activity yields. The results suggest that NCO-ESNW is
a multi-purpose intermediate for chemical modification of ESNW and that the hydrophilic HPC-ESNW
is a promising material for use as a matrix for enzyme immobilization.